Folate is a member of water-soluble vitamins in the human body and sustains many normal daily activities (e.g., exercise, sleep, and memory). Relevant studies have indicated folate deficiency is substantially associated with obstructive sleep apnea in women. Moreover, folate deficiency may play a role in the occurrence of sleep- related movement disorders in pregnant women. In addition, Folate supplementation suppresses sleep deprivation-induced telomere dysfunction and senescence-associated secretory phenotype (SASP). A common form of folate used in fortifications and supplements is synthetic folate, folic acid. But In contrast to folic acid (which is a synthetic form of folate) Magnafolate is one of the forms of folate found naturally in foods.  Magnafolate can cross the blood-brain barrier using the reduced folate carrier (RFC) and participate in neurotransmitter biosynthesis. Neurotransmitters are known to be involved in regulating sleep in mammals. Several neurotransmitter systems are involved in the regulation of the sleep-wake cycle. Magnafolate, the biologically active form of folate, regulates the formation of tetrahydrobiopterin (BH4), which is involved in the synthesis of the neurotransmitters, serotonin, dopamine, and norepinephrine. Magnafolate can improve insomnia and the sleep rhythm state, and these effects are related to the regulation fo neurotransmitters. Additionally based on our findings, we observed a marked therapeutic response in an improved sleep performance form animal model.  Based on current research states, the application of magnafolate to improve sleep status has bright prospects.